Olympus microscopes and photomicrographic equipment

Instruction booklet

Reproduced with permission from the copyright owner, Olympus Optical Co., Ltd

HOW TO USE THE PHOTOMICRO ADAPTERS L & H

As previously mentioned, the connecting units employed in the Photomicro Group can be divided into two groups, according to the total magnification. One group centering on the Photomicro Adapter L is chiefly used for total magnifications of 250× and lower; the other group, centering on the Photomicro Adapter H, for magnifications higher than 250×.

Whatever the magnification, to master photomicrographic techniques you must first become familiar with every unit. The following pages describe how to use the Photomicro Adapters L, H, Eyepiece Adapters PM-ADF, PM-ADP, PM-ADG-3 and Photomicro Stand PM-PSS.

USE OF THE ADAPTER L

• Function of the Adapter L

For photomicrography with the OM body, this adapter is used to mount a photo eyepiece on the camera. The eyepieces available for this use are the NFK (FK), P and G. Each of them requires a specific eyepiece adapter PM-ADF, PD-ADP or PM-ADG-3. For 20× objectives, with total magnification up to 250×, the Adapter L directly connects to either of these three eyepiece adapters. Note, however, that the Adapter L can be directly applied to the Olympus Universal Research Microscope VANOX, BH2 series microscopes and the Photomicrographic Stand PM-PSS for photomicrography with the OM body through the whole range of magnification.

• Use the Adapter L

1. Replace the standard Focusing Screen 1-13 with the Screen 1-12.

2. Mount the the Photomicro Adapter L to the OM body. The Adapter L is attached to the camera in the same manner as a normal interchangable lens.

3. Determine the magnification.

Picture magnification is determined by the following formula.

Eyepiece	Picture magnification
NFK (FK)	NFK (FK) eyepiece power × objective power
P & G	P or G eyepiece power × objective power × approx. 0.5

Photo eyepiece	Compatible microscope
NFK FK G P	VANOX (AHB-LB), BH2 VANOX (AH) & BH Stereoscopic Microscopes Others

4. In photomicrography, microscopes require special eyepieces FK, P or G for use with the OM body and Adapter L for low power magnification

• When either eyepiece FK or P is suitable, FK is preferable to P, since it is specially designed for photomicrography.
• Only the objective has resolving power. The eyepiece serves merely to enlarge the image resolved by the objective. For a given total magnification involving different combinations of eyepieces and objectives, the use of a higher power objective will result in finer resolution.

5. Select an eyepiece adapter suitable for the eyepiece in use.

• Eyepiece Adapter PM-ADF for Photo Eyepieces NFK (or FK)
• Eyepiece Adapter PM-ADP for Eyepieces P
• Eyepiece Adapter PM-ADG-3 for Eyepieces G

NOTE:
The VANOX (AH and BH series including AHB-LB), BH2 and PM-PSS do not require any eyepiece adapter.

6. Mount the Adapter L with camera onto the microscope.

After selection of the Eyepiece/Eyepiece Adapter combination, mount the entire camera assembly on the microscope as follows:

a. For Photo Eyepiece NFK (or FK)

1. Attach the Eyepiece Adapter PM-ADF to the photo tube of microscope, and clamp with screw provided.
2. Insert the selected Eyepiece NFK (FK) into the Eyepiece Adapter PM-ADF.
3. Fit the Adapter L over the Eyepiece Adapter PM-ADF pushing all the way down, and clamp the entire camera assembly.

b. For Eyepiece P

1. Fit the Eyepiece Adapter PM-ADP to the photo tube pushing all the way down.
2. Insert the Eyepiece P into the photo tube.
3. Pressing down the eyepiece against the photo tube slightly, raise the Eyepiece Adapter until it contacts with the eyepiece flange (be sure the eyepiece flange is in contact with both the photo tube and Eyepiece Adapter) and lock the adapter firmly with clamping screw.
4. Fit the Adapter L over the Eyepiece Adapter pushing all the way down and clamp the entire camera assembly.

c. For Eyepiece G10×

1. Unscrew the upper part of the Eyepiece Adapter PM-ADG-3.
2. insert the G10× into the lower part.
3. Replace the upper part of the Adapter.
4. Attach the Eyepiece/Eyepiece Adapter combination to the Adapter L, and clamp.
5. Insert the entire camera assembly into the microscope eyepiece sleeve pushing all the way down, and clamp.

d. For Eyepieces G15× and G20×

1. insert the G15× or G20× into the Adapter PM-ADG-3.
2. Attach the Eyepiece Adapter PM-ADG-3 to the eyepiece sleeve, and clamp.
3. Fit the Adapter L over the Eyepiece Adapter pushing all the way down, and clamp the entire camera assembly.

e. For Eyepieces GWH10×

1. Use the intermediate ring marked with letters “GWH 10×”, provided with the PM-ADG-3.
2. Unscrew the PM-ADG-3 into 3 parts.
3. Insert the Eyepiece GWH10× into the lowest ring with clamping screw.
4. Re-screw the other 2 rings into the lowest one.
5. Place the Adapter L on the PM-ADG-3 and clamp.
6. Connect the entire assembly into one of the eyepiece sleeves of the stereo microscope, and clamp.

7. Adjust the camera position.

If it is necessary to adjust the camera position, loosen the clamping screw provided on the Adapter L, rotate the camera to the desired position, and reclamp.

8. Focus.

Focusing with the OM System Focusing Screen 1-12.

1. Use the Dioptric Correction Lens or the Varimagni Finder, so that the double cross hairs within the field of view can be clearly recognized as two lines; otherwise correct focus cannot be obtained.
2. Adjust the coarse and fine adjustment controls of the microscope to bring the specimen into focus. Check again if both the double cross hairs and the specimen are equally sharp in focus.

9. Determine the exposure.

1. For the OM-1 and OM-2 in manual operation: Rotate the shutter speed ring or adjust light intensity by means of voltage adjustment of the light source or ND filters, until the needle in the viewfinder comes to the center of index marks.
2. For the OM-2 in automatic operation: You can use the automatic exposure control system built in the OM-2.
   * If the subject is darker than the background, turn the exposure ring or exposure compensation ring to over-expose by one or two F stops according to the situation.
   On the contrary, if the subject is brighter than the background, turn the ring to under-expose.

USE OF PHOTOMICROGRAPHIC STAND PM-PSS

To set up the microscope on the PM-PSS, as illustrated above, please read the instructions provided with the stand, and then proceed as follows:

1. Set the Focusing Screen 1-12 to the OM body.

2. Place the microscope on the base of the PM-PSS, so that the photo tube comes directly under the camera supporting arm.

3. Insert the eyepiece into the photo tube.

1. For Photo Eyepiece FK
   Put the light shield tube on the microscope photo tube, into which the selected Photo Eyepiece FK is inserted slowly.
2. For Eyepiece P
   Insert the selected Eyepiece P into the photo tube without the light shield tube.

4. Looking through the camera mounting hole in the arm, let down the arm until the Eyepiece enters the hole (if necessary, adjust the microscope position so as to accurately center the Eyepiece in the hole).

5. Loosening the clamping screw provided on the Adapter L, put the Adapter L over the camera mounting hole, and clamp.

6. Lower the arm slowly until it stops (at this height, the diaphragm inside the Adapter L touches the top of the eyepiece).
Then raise the arm by about 1mm so that the Adapter L floats off to the Eyepiece. Take care not to allow the periphery of the Eyepiece or light shield tube to come into contact with the edge of the camera mounting hole. Then raise the collar to support the arm.

7. Mount the OM body on the Adapter L.

8. Focus and expose.

OLYMPUS
OLYMPUS OPTICAL CO., LTD.
43-2 Hatagaya 2-chome, Shibuya-ku, Tokyo, Japan